Insertion junction: LMJ.RY0402.076254_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076254
Chromosome:chromosome 14
Location:867394
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g613400 POC16 Proteome of centriole protein 16; (1 of 1) PTHR13720//PTHR13720:SF27 - WD-40 REPEAT PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTCGCTCCCCTCCCCGCGCCTCTGCCTG
Internal bar code:CCGGAAATCCCGTACTTCGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:94.4958
LEAP-Seq n confirming:6850
LEAP-Seq n nonconfirming:399
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGACACCTGTGGTGAGG
Suggested primer 2:AGGCGTGTGTTAAAGAGCGT