| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076261 |
| Chromosome: | chromosome 2 |
| Location: | 354058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075650 | (1 of 1) K12872 - pre-mRNA-splicing factor RBM22/SLT11 (RBM22, SLT11) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCAAGTCGTCATGTCGTGTCATCCGAC |
| Internal bar code: | GCATCGATTCAATGCAAAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 66.3504 |
| LEAP-Seq n confirming: | 909 |
| LEAP-Seq n nonconfirming: | 461 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCAGCAGTAGGCGAGG |
| Suggested primer 2: | GCTACTTGGGCTGACTGGAG |