| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076300 |
| Chromosome: | chromosome 6 |
| Location: | 1666992 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261050 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCCACCCACCACTCCTCCCGCTCTTC |
| Internal bar code: | GGGTCGCACGGTCTTTGAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 97.6787 |
| LEAP-Seq n confirming: | 2104 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTTTGTTCCCCGCTTTA |
| Suggested primer 2: | CCTCCACACACACACACACA |