| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076311 |
| Chromosome: | chromosome 1 |
| Location: | 6060211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043050 | SNR2 | SNARE-associated Golgi protein; (1 of 2) PTHR12677:SF5 - TVP38/TMEM64 FAMILY MEMBRANE PROTEIN YDJX | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATCGTGATTTCCCTTGTTCACCCACTC |
| Internal bar code: | AGGGCTACTTAAGCCCGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.2509 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGGCCGTACAGGTAGT |
| Suggested primer 2: | TTAGCGTGAGCCGATTTTCT |