| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076311 |
| Chromosome: | chromosome_12 |
| Location: | 6145657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCCCCACCTGCGCACACGTGCAAGACAGG |
| Internal bar code: | CTGGGGGGGGTCGCATTACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 82.1134 |
| LEAP-Seq n confirming: | 42809 |
| LEAP-Seq n nonconfirming: | 9325 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGCATCTCACGGATGA |
| Suggested primer 2: | CCGCAACACACACACACATA |