| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076312 |
| Chromosome: | chromosome 9 |
| Location: | 1521135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397950 | (1 of 1) K05756 - actin related protein 2/3 complex, subunit 3 (ARPC3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCCCCTCCTGCGGACCGTCTGCACG |
| Internal bar code: | ACACCGCGTAAGATAGCGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 67.3333 |
| LEAP-Seq n confirming: | 1515 |
| LEAP-Seq n nonconfirming: | 735 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACGCTTTCCGCTTCTA |
| Suggested primer 2: | AGCCACGCACATAGGTATCC |