| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076341 |
| Chromosome: | chromosome 3 |
| Location: | 7484513 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205550 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCATGCGCTCGCAAGGACTCCGCCGCG |
| Internal bar code: | GAGAGAAATGCGAGTGACTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 71.0 |
| LEAP-Seq n confirming: | 284 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACTGGTACTCGCTGTG |
| Suggested primer 2: | GTACCGTGGCACTGATGTTG |