Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.076370 |
Chromosome: | chromosome 9 |
Location: | 2309798 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g392251 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGAAGGGTTTACATGCGGGGCAGTGAT |
Internal bar code: | GCCAAACCGGATGTCTAAATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 695 |
LEAP-Seq percent confirming: | 98.7288 |
LEAP-Seq n confirming: | 1165 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTAAGACTAAACGCGCA |
Suggested primer 2: | GCGTCGAGCTTTCTCTCTGT |