Insertion junction: LMJ.RY0402.076370_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.076370
Chromosome:chromosome 9
Location:2309798
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g392251 (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGAAGGGTTTACATGCGGGGCAGTGAT
Internal bar code:GCCAAACCGGATGTCTAAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:695
LEAP-Seq percent confirming:98.7288
LEAP-Seq n confirming:1165
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTAAGACTAAACGCGCA
Suggested primer 2:GCGTCGAGCTTTCTCTCTGT