| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076384 |
| Chromosome: | chromosome 12 |
| Location: | 2692993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503850 | ARLP2,ARL12 | ARF-like GTPase; (1 of 4) K07937 - ADP-ribosylation factor 1 (ARF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCCGATGGTGGGGATGGTCGTGACTA |
| Internal bar code: | GGGGGGATGCCGATGGTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 73.7778 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACGATTGATGAGGTGA |
| Suggested primer 2: | GCGTGACCTTCTTCTTCGTC |