| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076396 |
| Chromosome: | chromosome 2 |
| Location: | 5330580 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107000 | (1 of 1) PTHR24056//PTHR24056:SF11 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGCAGCTAGCAGCCTGGTTTGGTCACG |
| Internal bar code: | AGTCGGACGTCGCCGGCGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 94.1606 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAAGTCGGACTGCGAGGT |
| Suggested primer 2: | CTCAGCACCGCACAAAAATA |