| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076407 |
| Chromosome: | chromosome 11 |
| Location: | 1666555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467774 | (1 of 1) IPR000104//IPR003409 - Antifreeze protein, type I // MORN motif | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTCCGTCGCCTCTGTGCTTCGCCTCTG |
| Internal bar code: | AACCCCCCGTTTTTCTTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACATGGTGTATGCGGG |
| Suggested primer 2: | CATACGTACACGTTTTGGCG |