| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076430 |
| Chromosome: | chromosome 14 |
| Location: | 1068220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614850 | FAP33,ANK17 | Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGGTTCGTTGGCGTCCAAGCGTGGCG |
| Internal bar code: | TCCGACTCAACCGGTGGCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.726 |
| LEAP-Seq n confirming: | 728 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCTACTAGCCCAGCGCAA |
| Suggested primer 2: | GCCCTGACAAGCTTTCACTC |