| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076436 |
| Chromosome: | chromosome 1 |
| Location: | 4761314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032900 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGACTCAGATGCATTACCGGTACACTCG |
| Internal bar code: | TCTTAACCGAAGTGCGGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 97.8788 |
| LEAP-Seq n confirming: | 8998 |
| LEAP-Seq n nonconfirming: | 195 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATGACAAGGACACGCT |
| Suggested primer 2: | AACCCATGATAAAATGCCCA |