| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076436 |
| Chromosome: | chromosome 12 |
| Location: | 7848144 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553050 | NUP53 | (1 of 1) K14313 - nuclear pore complex protein Nup53 (NUP35, NUP53); Nucleoporin 53 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGCCACGCCTGCCGGAAGTATGTTAC |
| Internal bar code: | AAACGCATGTAACTAGTTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 92.9539 |
| LEAP-Seq n confirming: | 343 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTGGAGGAGGAGAGCG |
| Suggested primer 2: | ACCGCCCCTAGTCTACACCT |