| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076468 |
| Chromosome: | chromosome 7 |
| Location: | 330257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314500 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACCTGGCACGCAGCAGCGCGCGCTCCA |
| Internal bar code: | CGAGAGAAACGAAGGCAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 93.0851 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGCGGCTTACGAAGAAC |
| Suggested primer 2: | TCAATGTCCTGCTGCACTTC |