| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076488 |
| Chromosome: | chromosome 7 |
| Location: | 1301861 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322000 | CSD2,NIFS2 | (1 of 1) PTHR11601//PTHR11601:SF20 - CYSTEINE DESULFURYLASE // SELENOCYSTEINE LYASE; Cysteine desulfurase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGGGGCGTTGCAAAGATTGGTAGAGA |
| Internal bar code: | GCGATTAAGTAGTATCGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2232 |
| LEAP-Seq percent confirming: | 93.262 |
| LEAP-Seq n confirming: | 71088 |
| LEAP-Seq n nonconfirming: | 5136 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | CATCCGCTCCACTACCGTAT |