| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076489 |
| Chromosome: | chromosome 11 |
| Location: | 2869490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477750 | (1 of 1) IPR016201 - Plexin-like fold | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGTTCATCCATTTGGCATTGCTGAAA |
| Internal bar code: | GGCATCCGGGAAAGCCCGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 99.835 |
| LEAP-Seq n confirming: | 5446 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTAGATGACGCAGTGGA |
| Suggested primer 2: | TTATGTTCCGTTGGTGCGTA |