Insertion junction: LMJ.RY0402.076491_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.076491
Chromosome:chromosome 17
Location:3577686
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g725600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACCGGAGACCCTTTCTCCTCCTGGACC
Internal bar code:GCAGGGATAGGCTTGAGCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:98.3784
LEAP-Seq n confirming:182
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGCTGCACCTGGTTAAA
Suggested primer 2:CGAGTCACCGACACACTCAT