| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076533 |
| Chromosome: | chromosome 12 |
| Location: | 9554901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546452 | GT90F45,GT90-45 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGCCGGGGCTGACGGCAAGAGGTAGC |
| Internal bar code: | CCCCGGCGTTCGTAGATCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.5485 |
| LEAP-Seq n confirming: | 5071 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCGTTAATGCTTGACCA |
| Suggested primer 2: | ACTACGTGCCGTTCTGGTCT |