| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076560 |
| Chromosome: | chromosome 1 |
| Location: | 5757691 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAGACGGGGAGAGCGGGTGCGGTGGGC |
| Internal bar code: | GTGGTCTACCTCCCCTATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 94.4816 |
| LEAP-Seq n confirming: | 565 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCATCGCTGAGCTCGT |
| Suggested primer 2: | GCTTTTTGGTTTGCGTTGAT |