| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076561 |
| Chromosome: | chromosome 3 |
| Location: | 1761363 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153950 | TRP12 | Transient receptor potential ion channel protein; (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGACCCCGGTCACAGCCTACCACAGCT |
| Internal bar code: | CGATATCGTACGCGAAGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 99.9248 |
| LEAP-Seq n confirming: | 1328 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGCGTGTGTCTTCACTC |
| Suggested primer 2: | CTGCCGATGCAGTAGTCAAA |