| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076569 |
| Chromosome: | chromosome 1 |
| Location: | 1516124 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007811 | (1 of 1) PF15383 - Transmembrane protein 237 (TMEM237) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACTTGGGTCCGTAAGTGTCACGTCT |
| Internal bar code: | TCAACGCCTACATTCTCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 99.6194 |
| LEAP-Seq n confirming: | 12040 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTGTGGAGGAGGTGT |
| Suggested primer 2: | GGGTAGTGACACGGGTATGG |