| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076580 |
| Chromosome: | chromosome 7 |
| Location: | 2713846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331114 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGCATCCTATCTGGCTAGCTGCGGTT |
| Internal bar code: | AAAAAGATACTTAATTCGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 98.0998 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTCTATCCGGCATTGCT |
| Suggested primer 2: | CTACGACTGGAGCAATGCAA |