Insertion junction: LMJ.RY0402.076586_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.076586
Chromosome:chromosome 10
Location:3644724
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g445650 SMC3 (1 of 1) K06669 - structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6) (SMC3, CSPG6); Structural Maintenance of Chromosomes protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGTCAATGGGCACCGCTTTGGTGGGATT
Internal bar code:CTGGGCCAGCCTGAACCTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:566
LEAP-Seq percent confirming:96.0069
LEAP-Seq n confirming:1106
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTTGGCATTCCAGTGTC
Suggested primer 2:ACCCTAGAGCGCACAAGAAA