| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076605 |
| Chromosome: | chromosome 3 |
| Location: | 9145211 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212081 | (1 of 3) IPR013328 - 6-phosphogluconate dehydrogenase, domain 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACAGCTGATCGCAGTTCCTCGCCGGT |
| Internal bar code: | GGGTGCCTTACAGCGTCATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 2128 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATGCTGTTGCTGATGT |
| Suggested primer 2: | AAATGGTAGTCGGCTTGGTG |