| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076606 |
| Chromosome: | chromosome 4 |
| Location: | 1846620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211600 | snRK1,CKIN1,SNF1,SNRK1A | (1 of 1) PTHR24343//PTHR24343:SF183 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTACATGGTTATAATAGACAATGCCGCC |
| Internal bar code: | AGTATTGAGGGAGGATCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 90.8784 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATCATAACCCTCCTCC |
| Suggested primer 2: | TTGTCCGCACACCACATACT |