| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076609 |
| Chromosome: | chromosome 3 |
| Location: | 333560 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144707 | (1 of 1) 5.4.2.2//5.4.2.8 - Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) / Phosphoglucose mutase // Phosphomannomutase / Phosphomannose mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACTGTCCGTATGATCCACACACCTCAC |
| Internal bar code: | GCGCGTGGTCCTTCTACAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 89.4309 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTTCTTCTCCACTGCT |
| Suggested primer 2: | GCATGAACACAGCCAAGAAA |