| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076617 |
| Chromosome: | chromosome 5 |
| Location: | 567923 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTGCGAGTTCTTTGGAGGGGATCGAG |
| Internal bar code: | CTCAAATGGGAATGTACGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACAGGATGCCCTGAAGA |
| Suggested primer 2: | ACCAAACCAAACCAAACCAA |