| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076635 |
| Chromosome: | chromosome 13 |
| Location: | 949410 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568150 | CMAL1,SMM45,CMAL | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.199 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTTGCTGACAGGCTGGCGCGTGGGGCG |
| Internal bar code: | CTGGGCGGGAACTACCCGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 99.7992 |
| LEAP-Seq n confirming: | 497 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCCTTCCAGTCTCCAC |
| Suggested primer 2: | TTAAACATCCTCGCAAACCC |