| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076681 |
| Chromosome: | chromosome 2 |
| Location: | 1985806 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088300 | CPK6 | (1 of 1) PTHR10584:SF179 - PROTEIN MAK32; putative carbohydrate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGACTGCGGTGGCAGACAGAGGAGATG |
| Internal bar code: | CAGAGACTATGGCCGCGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.6658 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCTCGATGAAGGTGCT |
| Suggested primer 2: | CTCCTGGGTCGACTCATTGT |