| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076707 |
| Chromosome: | chromosome 4 |
| Location: | 3399034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227526 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGACAGGCAGCTGCTGTGCACCCTTGC |
| Internal bar code: | ATTACGCGCTCACGATGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.7365 |
| LEAP-Seq n confirming: | 11355 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGTTCAGCGGTAAGGT |
| Suggested primer 2: | TTGTGCTCTTCGAGGAAGGT |