| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076728 |
| Chromosome: | chromosome 3 |
| Location: | 3655886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168850 | (1 of 1) K17434 - large subunit ribosomal protein L53 (MRPL53) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACACGACCTTCCGGGCTGGGACTGTA |
| Internal bar code: | TCTGGCACGGGCGTGCCCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.2611 |
| LEAP-Seq n confirming: | 1209 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACCCAGCACATATCAAA |
| Suggested primer 2: | CTACCCGCTGCAAATAGAGC |