| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076738 |
| Chromosome: | chromosome 4 |
| Location: | 1346551 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214500 | IDH3 | (1 of 1) 1.1.1.42 - Isocitrate dehydrogenase (NADP(+)) / oxalosuccinate carboxylase; Isocitrate dehydrogenase, NADP-dependent | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTGGTTAGCGCAAGTTCTCGGACGCCG |
| Internal bar code: | TCGCGCATTTAAACGTGAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 99.4137 |
| LEAP-Seq n confirming: | 3900 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTGCTCATCCTAAAC |
| Suggested primer 2: | TCACCACCAACGACCAACTA |