| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076741 |
| Chromosome: | chromosome 12 |
| Location: | 8552246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCATAATCCAGTCCACCGTGGAATGAA |
| Internal bar code: | GCATCATGGGTGGAATCGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.9316 |
| LEAP-Seq n confirming: | 1460 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTGGCTTGTGCATCTAA |
| Suggested primer 2: | GTTTGTGCAGGTCAGGGATT |