| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076753 |
| Chromosome: | chromosome 7 |
| Location: | 2508617 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329550 | VFL5 | Variable flagella protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAATGATGAGGTGGGTGTTGCCGGGCCC |
| Internal bar code: | GACAACACTCGTTTTATCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 75.9887 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 340 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCAGTACACCAGGCAA |
| Suggested primer 2: | CGGAATTACGGTGTTTTGCT |