Insertion junction: LMJ.RY0402.076774_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.076774
Chromosome:chromosome 2
Location:881617
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g079300 VPS4 AAA-ATPase of VPS4/SKD1 family; (1 of 1) K12196 - vacuolar protein-sorting-associated protein 4 (VPS4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTCTGACGCAACGCTAAACGATAGGAAC
Internal bar code:TGAAACCATGCGCTAGTTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:706
LEAP-Seq percent confirming:98.5997
LEAP-Seq n confirming:2394
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGGCTAGGACTCACTG
Suggested primer 2:CTCTGGACTTGGAGGCTGAG