| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076808 |
| Chromosome: | chromosome_3 |
| Location: | 2375682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g158750 | HEL14 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCCGCAGCCCCTCGCCGGCTGAACCTCT |
| Internal bar code: | GCAAGCGAGTAGCTCGGTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.2848 |
| LEAP-Seq n confirming: | 1527 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAGCACCTTCATCACA |
| Suggested primer 2: | CGACAATCTGCTCTGTGCAT |