Insertion junction: LMJ.RY0402.076810_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076810
Chromosome:chromosome 10
Location:4507452
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g452000 (1 of 1) PF04752 - ChaC-like protein (ChaC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGACACAGGGTGTTGTATTTTGGTATT
Internal bar code:TTCGCACCGGCAGGGAACGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:127
LEAP-Seq percent confirming:6.8306
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:1023
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCACCACTCAGCGCAATA
Suggested primer 2:CCATGCCCACATGTCTACAG