| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076831 |
| Chromosome: | chromosome 6 |
| Location: | 307081 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251150 | OHP2,OHP1,LIL6,LCI25 | One-Helix Protein 2; (1 of 45) IPR023329 - Chlorophyll a/b binding protein domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAACGCAACTGTACAACATGTCCTTTT |
| Internal bar code: | CGGATCGGCGTTGGTTAGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 81.0712 |
| LEAP-Seq n confirming: | 1105 |
| LEAP-Seq n nonconfirming: | 258 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGACGTAGAAGGCTTGC |
| Suggested primer 2: | CCGATATGCAGCAGTCTTCA |