| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076842 |
| Chromosome: | chromosome 4 |
| Location: | 201289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217150 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCAGGTTGTAGTCTGGGGGCACGCGCT |
| Internal bar code: | TATTGTCTAACTGGGCTCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.2481 |
| LEAP-Seq n confirming: | 2244 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTGGTCTTTTTAATGCG |
| Suggested primer 2: | ATCGCTGTAAGTGTTTGGGG |