Insertion junction: LMJ.RY0402.076850_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.076850
Chromosome:chromosome 7
Location:2203931
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g327150 (1 of 2) IPR001810//IPR003347 - F-box domain // JmjC domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGTGCGCACTTTCAGGAGCACAATGAAG
Internal bar code:CGTACGCATCCCGGTCCACCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:622
LEAP-Seq percent confirming:99.701
LEAP-Seq n confirming:5336
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACACGGGCAATTAAACC
Suggested primer 2:GGCTATAGGGCTGGAGAAGG