| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076855 |
| Chromosome: | chromosome 8 |
| Location: | 4865002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384750 | AMYA3,AMA3 | Alpha-amylase 3; (1 of 3) K01176 - alpha-amylase (E3.2.1.1, amyA, malS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAGGTGTGTCGCTGGCACCCTAATCGAG |
| Internal bar code: | GTCGTCGACAGCGCCCGAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.0028 |
| LEAP-Seq n confirming: | 4858 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAAGTGAGTGGGGCTGA |
| Suggested primer 2: | CCACCAATCTCTCCAGTGGT |