Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.076863 |
Chromosome: | chromosome_9 |
Location: | 1913041 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g395250 | FAP36 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGCTCTGCCCCCGCGCACCCCCGCCCGTG |
Internal bar code: | GAACAGGTTAGGAGTGGCGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 549 |
LEAP-Seq percent confirming: | 66.2517 |
LEAP-Seq n confirming: | 1490 |
LEAP-Seq n nonconfirming: | 759 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCAGACAGCCCAATAACCG |
Suggested primer 2: | CAACCCCTCCACATCATACC |