| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076866 |
| Chromosome: | chromosome 12 |
| Location: | 7655275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554550 | (1 of 1) IPR026771 - Transmembrane protein 218 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCCATCCGCCGTGAGCACATGCCACTC |
| Internal bar code: | TAACCGTTGAAGGCGGCCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 97.1104 |
| LEAP-Seq n confirming: | 2823 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTCTGGTTGTAGCCCTG |
| Suggested primer 2: | TTTGCAAGCTGTCCAGTCAC |