| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076873 |
| Chromosome: | chromosome 1 |
| Location: | 2687443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015950 | CPL11 | (1 of 1) PTHR17490//PTHR17490:SF7 - SUA5 // SUBFAMILY NOT NAMED; translation factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACCGGAACGCTTTCTGGCCGGATAACT |
| Internal bar code: | AATCAGCGCCTCTAGTTCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.6185 |
| LEAP-Seq n confirming: | 7834 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAGAGTTCATCCGTCCC |
| Suggested primer 2: | CATGAAGTCAATGCACTGGG |