| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076874 |
| Chromosome: | chromosome_9 |
| Location: | 5134337 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g398882 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGTGTTCGATGGCTCCAGTTGCGTCTCAG |
| Internal bar code: | GTGGGAGAGTAGACGAAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 97.361 |
| LEAP-Seq n confirming: | 5165 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCAGTACAAGGAGGAG |
| Suggested primer 2: | GCACAGTAGTGGTGCCTTGA |