Insertion junction: LMJ.RY0402.076885_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.076885
Chromosome:chromosome 6
Location:1901130
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g263289 (1 of 1) 2.5.1.87 - Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific) / Rer2p Z-prenyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTCGACTGACACGCCGCCTTCGGCCCGT
Internal bar code:GGTTGGGGCCATGTCTGCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:98.9933
LEAP-Seq n confirming:885
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGCTCCCACAGAGTAGG
Suggested primer 2:AATCGTGTAATCGCCCTGAC