Insertion junction: LMJ.RY0402.076886_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076886
Chromosome:chromosome 7
Location:6140022
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g355800 (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTTCAACCCTGTCCCATTGCACACACA
Internal bar code:GGGTGGGGCACGAGGGAGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:96.3143
LEAP-Seq n confirming:1385
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGCATTTCGACTTCGGCT
Suggested primer 2:GGGCTGTCTCATAACTCCGA