Insertion junction: LMJ.RY0402.076899_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.076899
Chromosome:chromosome 16
Location:4220398
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g668552 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCCGGCTTTCGATCAGCCACGCGCTCA
Internal bar code:CTCCCCCGTCGACCGGCAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:429
LEAP-Seq percent confirming:83.0207
LEAP-Seq n confirming:841
LEAP-Seq n nonconfirming:172
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACGAGGGTTTGTGAATGTG
Suggested primer 2:ATCGACTTCTTCAACCCCCT