| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076918 |
| Chromosome: | chromosome 9 |
| Location: | 3436550 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389245 | (1 of 1) PTHR24078//PTHR24078:SF205 - DNAJ HOMOLOG SUBFAMILY C MEMBER // DNAJ HEAT SHOCK N-TERMINAL DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCTGGATCCAGGTCGTCCAGGTCGTCG |
| Internal bar code: | CGTTCCTCGGTCCCGGTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 151 |
| LEAP-Seq percent confirming: | 99.4007 |
| LEAP-Seq n confirming: | 1327 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTCTGGATGAGGTAGGC |
| Suggested primer 2: | CACCGAATAGGACTGGCATT |